Concentrations of extracted dried sample were analyzed on spectrophotometer at 420 nm with respect to standard graph (fig 3). We got regration values such as 0.998, 0.997, 0.986, and 0.991 for methanol (with conc. 60μg/ml), acetone (100μg/ml), ethyl acetate (200μg/ml) and chloroform (100μg/ml) respectively.