Mucilage extraction was performed at different seed:distilled water ratios, pH and temperature conditions. A Box Behnken experimental design (BBD) was used for the evaluation of mucilage extraction conditions that included 30 experimental runs with three center points at high and low levels of each independent variable:temperature (20–80 °C), pH (4–8) and seed:water ratio (1:20; 1:40). The response (dependent variable) was the extraction percentage under the different conditions.
Samples of 10 g of whole seeds were placed in a 1 L beaker and distilled water was added in 1:20, 1:30 and 1:40 proportions. The pH was adjusted and maintained at 4, 6 and 8 through continuous adjustments using 0.2 M NaOH or HCl solutions and the temperature during extraction was maintained at 4, 40 and 80 ± 1.5 °C using temperature controller. The mixtures were stirred with a magnetic stirrer and hydrated for 2 h. Then the aqueous suspension was spread on a drying tray and exposed to temperature of 50 °C for 10 h. The dried mucilage was separated from the seed by rubbing over a 40 mesh screen, and the weight was recorded.
Mucilage extraction was performed at different seed:distilled water ratios, pH and temperature conditions. A Box Behnken experimental design (BBD) was used for the evaluation of mucilage extraction conditions that included 30 experimental runs with three center points at high and low levels of each independent variable:temperature (20–80 °C), pH (4–8) and seed:water ratio (1:20; 1:40). The response (dependent variable) was the extraction percentage under the different conditions.Samples of 10 g of whole seeds were placed in a 1 L beaker and distilled water was added in 1:20, 1:30 and 1:40 proportions. The pH was adjusted and maintained at 4, 6 and 8 through continuous adjustments using 0.2 M NaOH or HCl solutions and the temperature during extraction was maintained at 4, 40 and 80 ± 1.5 °C using temperature controller. The mixtures were stirred with a magnetic stirrer and hydrated for 2 h. Then the aqueous suspension was spread on a drying tray and exposed to temperature of 50 °C for 10 h. The dried mucilage was separated from the seed by rubbing over a 40 mesh screen, and the weight was recorded.
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