Broodstock were collected in the Strait of Georgia, BC, off Denman
Island in October 2010 and in Kulleet Bay, BC in October 2011, and
had a mean shell length and live weight of 157.7 ± 1.9 cm and
1.28 ± 0.05 kg (±SE, n = 47) and 149.6 ± 2.1 cm and 1.40 ± 0.04 kg
(n=50), respectively. Approximately 25 animalswere laid horizontally
on the bottomof a shallowtank (L ×W× H: 1.2 × 0.9 × 0.3m) and provided
with flow-through, sand-filtered, and UV-treated seawater at
3–4 l min−1 and 8–12 °C. A single-algal diet of CM or TL was drip-fed
at a daily ration of 4–6 × 109 cells ind.−1. Spawning was induced by
adding excessive TL to the holding tank. Fertilized eggs obtained from
multiple parents were hatched in shallow tanks (L × W × H:
1.2 × 0.9 × 0.3 m) at a density of b30 eggs ml−1 and a temperature of
12–15 °C. After 48–60 h, newly developed D-larvae were collected and
used for various experiments or for further rearing to produce juveniles,
as described below
Broodstock were collected in the Strait of Georgia, BC, off DenmanIsland in October 2010 and in Kulleet Bay, BC in October 2011, andhad a mean shell length and live weight of 157.7 ± 1.9 cm and1.28 ± 0.05 kg (±SE, n = 47) and 149.6 ± 2.1 cm and 1.40 ± 0.04 kg(n=50), respectively. Approximately 25 animalswere laid horizontallyon the bottomof a shallowtank (L ×W× H: 1.2 × 0.9 × 0.3m) and providedwith flow-through, sand-filtered, and UV-treated seawater at3–4 l min−1 and 8–12 °C. A single-algal diet of CM or TL was drip-fedat a daily ration of 4–6 × 109 cells ind.−1. Spawning was induced byadding excessive TL to the holding tank. Fertilized eggs obtained frommultiple parents were hatched in shallow tanks (L × W × H:1.2 × 0.9 × 0.3 m) at a density of b30 eggs ml−1 and a temperature of12–15 °C. After 48–60 h, newly developed D-larvae were collected andused for various experiments or for further rearing to produce juveniles,as described below
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