The protocol consists of two main parts: (i) electromyographic (EMG) and video recording and (ii) computer-assisted kinematics and EMG analyses. The minimum time required to complete the first part is about1.5–2.5 h. The variability is due mainly to how quickly thefish recover from anesthetics and are ready to respond to display stimuli. The second part can be performed offline. A typical analysis session generally requires 1–2 h.