Pasteurised potting mix in 10-cm-diameter plastic pots was inoculated with a mixture of the four isolates of P. erythroseptica alone, P. erythroseptica plus T. virens
DAR 74290, P. erythroseptica plus Trichodex, prepared as above, or left uninoculated, and 10 seeds of tomato cv. Grosse Lisse (Grower’s Pride, Hortico
(Aust.) Pty Ltd.) were sown in each pot. There were four replicate pots per treatment. Plants were maintained in the glasshouse as above, except that they
were set out in a completely randomised block design. Survival of seedlings was assessed at 10 day intervals but only data collected 30 days after inoculation
were analysed. Surviving seedlings were removed from pots after 30 days and symptoms of disease caused by P. erythroseptica were recorded using a 1–5 scale modified from Nemec et al. (1996). P. erythroseptica was re-isolated from the diseased tomato plants as described above.