Routine Formalin Fixation
1.“10%” Formalin consists of 10% by volume dilution of commercial
formalin in 0.1 M sodium phosphate buffer (pH 7.4 to 7.6) and is
prepared by dilution of 37% commercial formalin into buffer (final
concentration 3.7% by weight).
2. Fixation is from 24 h to several days, depending on size of tissue
block (for example, 24 h for ca. 500 mg mouse brain).