2.4.1. Extraction of phenolic compounds
At the end of fermentation (day 90) fromeach treatment samples of
ten olives were pitted, the pulp was frozen and then freeze-dried. The
drymasswas pounded in a mortar, and 1 g of the powderwas extracted
three times with 15 ml methanol. The extracts were combined, and
methanol was evaporated under a vacuum at 45 °C. The residue from
the methanol extraction was dissolved in 10 ml distilled water and
then shaken with hexane to remove lipids before extraction with
ethyl acetate. The lipid-free ethyl acetate solution was evaporated
under a vacuum at 70 °C. The residue was dissolved in 5 ml methanol.
The extract obtainedwas used for total phenol determination and identification
of the main biophenols of olives.