randomly assigned to each diet. 24 groups each of 25 fish weighing 13.45 ± 0.04 g fish−1 (mean ± S.D., n= 25).
The feeding trial lasted 12 weeks, during which the fish were fed to satiation thrice daily (09:00, 13:00 and 17:00 h), 7 days per week. At each feeding, an excess amount of feed was fed to fish and uneaten feed were collected 1 h after
feeding, dried at 70 °C and reweighed. Leaching of uneaten feed was estimated by placing weighed samples of each diet into a tank without fish for 1 h and then recovered, dried and reweighed. The average leaching value was used to correct
the amount of uneaten feed.
randomly assigned to each diet. 24 groups each of 25 fish weighing 13.45 ± 0.04 g fish−1 (mean ± S.D., n= 25).The feeding trial lasted 12 weeks, during which the fish were fed to satiation thrice daily (09:00, 13:00 and 17:00 h), 7 days per week. At each feeding, an excess amount of feed was fed to fish and uneaten feed were collected 1 h afterfeeding, dried at 70 °C and reweighed. Leaching of uneaten feed was estimated by placing weighed samples of each diet into a tank without fish for 1 h and then recovered, dried and reweighed. The average leaching value was used to correctthe amount of uneaten feed.
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