A potent cellulose producer, Acetobacter xyZinum BRCS, was cultivated in shaking flasks and jar fermeutors
with glucose, fructose, sucrose and mixtures of these as carbon sources. It was confirmed that corn steep liquor
(CSL) was cheap and a suitable organic nitrogen source for cellulose production by strain BRCS. When glucose
was used as a sole carbon source, strain BRCS oxidized almost all the glucose to gluconic acid, thereafter it
biosynthesized cellulose by utilizing the gluconic acid accumulated in the broth under limited glucose conditions.
Since strain BRCS did not metabolize fructose to acid, the fermentation pattern of fructose was found
to be typically growth associated with cellulose production. However, when glucose and fructose coexisted in
the medium, strain BRCS preferentially metabolized all the glucose to gluconic acid. Thereafter, it produced
cellulose mainly by utilizing fructose. Overall cellulose productivity in a jar fermentor ranged from 0.071 lo
0.086 g/l/h.
A potent cellulose producer, Acetobacter xyZinum BRCS, was cultivated in shaking flasks and jar fermeutorswith glucose, fructose, sucrose and mixtures of these as carbon sources. It was confirmed that corn steep liquor(CSL) was cheap and a suitable organic nitrogen source for cellulose production by strain BRCS. When glucosewas used as a sole carbon source, strain BRCS oxidized almost all the glucose to gluconic acid, thereafter itbiosynthesized cellulose by utilizing the gluconic acid accumulated in the broth under limited glucose conditions.Since strain BRCS did not metabolize fructose to acid, the fermentation pattern of fructose was foundto be typically growth associated with cellulose production. However, when glucose and fructose coexisted inthe medium, strain BRCS preferentially metabolized all the glucose to gluconic acid. Thereafter, it producedcellulose mainly by utilizing fructose. Overall cellulose productivity in a jar fermentor ranged from 0.071 lo0.086 g/l/h.
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