PCR is the amplification of a specific sequence of DNA so that it can be analyzed by scientists. Amplification is important, particularly when it is necessary to analyze a small sequence of DNA in quantities that are large enough to perform other molecular analyses such as DNA sequencing. Not long after PCR technology was developed, genetic engineering of DNA through recombinant DNA technology quickly became possible. Recombinant DNA is DNA that has been altered using bacterial derived enzymes called restriction endonucleases that act like scissors to cut DNA. The pattern that is cut can be matched to a pattern cut by the same enzymes from a different DNA sequence. The sticky ends that are created bind to each other and a DNA sequence can therefore be inserted into another DNA sequence.
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