Simmon's citrate agar medium was prepared and sterilized at 15 lbs for 15 min and poured as slants. These slants were streaked with the culture and were incubated at 37 °C for 24 h. The citrate test is used to determine the ability of a bacteria to utilize a soul source of carbon. Bacteria can break the conjugate base the salt of citrate into organic acids and carbon dioxide. Carbon dioxide can combine with sodium from conjugate basic salt to form basic compound sodium carbonate a pH indicator. Bromothymol blue in the medium detect presence of this compound by turning blue.