2.2. Crossing and F1 hybrids
‘UC-01’ and ‘Hawaii-30’, ‘Whiteberry’ and ‘Akihime’ were
crossed as shown in Table 2. For hybridization, each accession or
cultivar was clonally propagated from runner and planted in 2 L
plastic pots with compost soil. Pollen was collected from the male
parent and kept at 4 ◦C with a desiccator until used. Seed parents
were emasculated at the ‘white bud’ stage, and the emasculated
receptacle was covered with oiled paper to protect it from other
pollen. The emasculated receptacles were pollinated and the oiled