consequence of the cryo-concentration of salts and the confinement
of the particles in a small volume of unfrozen aqueous phase,
thus, the aggregation processes prevail. Due to the low oil mass
fraction (fm ¼ 0.025), protein aggregation play a preponderant role
in the destabilization of SB after freeze-thawing. An observation
made by Yeh et al. (1981) provides some support. In that work, Illinois
SB prepared by high pressure homogenization without calcium
salts addition were centrifuged at low speed and a fiber-rich
debris was separated. Then, freeze-thaw stability of noncentrifuged
and centrifuged SB was comparatively evaluated