Working standards for LC–DAD analysis were prepared by
diluting the stock solution of acrylamide to concentrations
of 0.1, 0.2, 0.3, 0.5, 1.0 and 2.0 g/ml with distilled water.
Stock solutions and working standards were kept at 4 ◦C for
a month. Carrez I solution was prepared by dissolving 15 g
of potassium hexacyanoferrate in 100 ml of water, and Carrez
II solution by dissolving 30 g of zinc sulfate in 100 ml of
water.