The mixture of vesicles forming ingredients
like surfactant and cholesterol are dissolved in
a volatile organic solvent (diethyl ether,
chloroform or methanol) in a round bottom
flask. The organic solvent is removed at room
temperature (20°C) using rotary evaporator
leaving a thin layer of solid mixture deposited
on the wall of the flask. The dried surfactant
film can be rehydrated with aqueous phase at
0-60°C with gentle agitation. This process
forms typical multilamellar niosomes. (Fig 2)