The mobile phases were composed of water (solvent A) and acetonitrile (solvent B) at a constant flow rate of 6 mL/min. The gradient started with 20% solvent B to 30% B in 30 min, 55% B in 35 min, and 20% B in 40 min
The mobilephases were composed of water (solvent A) and acetonitrile (solventB) at a constant flow rate of 6 mL/min. The gradient startedwith 20% solvent B to 30% B in 30 min, 55% B in 35 min, and 20%B in 40 min