Findings indicated that using 40 g/l sucrose in the rooting medium encouraged the adventitious
roots production where 5 roots per plantlet of 7.8 cm long and 1.4 mm width were averagely induced. The average leaves
number reached 3-4 leaves per plantlet, leaf length 18-28 cm and the leaf width 3.5-6.9 mm. Full strength of MS basal salts
proved better than macro elements of B5 & micro elements of MS. The leaves and roots growth was affected by the genotype
since the shoots of var. Gajar were initiated before other two varieties and in higher number. Incorporation of 3.0 g/l AC to
the rooting medium found to be necessary to enhance the whole plantlet growth as the length of white roots and leaves
increased. AC addition encouraged the secondary and tertiary roots formation. By adjusting factors affected rooting, the ideal
in vitro roots have been achieved within a short period of time (4 months) and the survival percentage of ex vitro plants
exceeded 90%. The in vitro roots development and proper handling of the plantlets during rooting stage was described in
current study.