An RT-PCR product amplified using primers flanking the YHV5f:
YHV5r primer pair (i.e., T7YHVu5f 5′-TAATACGACTCACTATAGGGATAT
CGTCCCGGCAATTGTG-3′ and YHVd5rv2 5′-TTCTAGCCATGCGGTGTT
GA-3′) was used as a DNA template to transcribe a synthetic RNA
using an in vitro Transcription T7 Kit according to the
manufacturer's instructions.