Retention time of H2 treatment on sperm motility
The retention time of motile sperm treated with H2 is an
important indicator of IVF and ICSI success. After treatment
with H2- or N2-mixed gases for 30 min, 10 sperm
suspensions were kept at 25°C in room air. After 2.5 h, we
found that forward motility in H2 treated suspension was
still higher than that before treatment (Figure 4). It is
noteworthy that H2 quickly diffuses. Approximately
10 min after placing an aliquot of the H2-treated sperm
suspension in room air, the dissolved H2 concentration
reached < 0.1% of its saturated concentration, indicating
that the higher sperm motility enhanced by H2 treatment
can be maintained without H2 for at least 2.5 h.