Classification of proteases by inhibitory studies
The major classes of proteases present in arowana enzyme extract were characterized
using selected specific protease inhibitors. The list of inhibitors was as follows: 10 mM
phenylmethylsulfonyl fluoride (PMSF; FlukaR Chemicals) in ethanol for serine proteases
inhibition, 10 mM tosyl-lysine chloromethyl ketone (TLCK; SigmaR) in 1 mM