Thrombin playstheroleincardiovascul

Thrombin playstheroleincardiovasculardiseasesandregulatesmanyprocessesininflammation and
could beafeatureofmanypathologicalconditions,includingthethromboembolicdisease,cancerand
neurodegenerativediseases.Anultrasensitiveandamplified electrochemicalsandwichassayusing
screen-printedcarbonelectrode(SPCE)stripsforthrombindetectionwasestablishedinthisstudy.The
conductivity andsensingperformanceofthecarbonelectrodeswereenhancedbyusinggold
nanoparticles (AuNPs).Theaptameraddressedonthestripswasusedasaprimaryprobetocapture
thrombin inthedetectedsamples.Anamplifier wasinventedforrecognizingthrombincapturedonthe
SPCE, whichisthemultiplemoleculesofanti-thrombinantibody(Ab)andhorseradishperoxidase(HRP)
co-modified AuNPs(AuNPs/Ab–HRP). HydrogenperoxidewasusedasthesubstrateforHRPandthenthe
response current(RC)couldbedetected.TheoptimizationoftheseAuNPsconjugates-amplified aptamer
immunosensing SPCEstripswasconductedforthrombindetection.Thedetectionsensitivityshoweda
linear relationbetweenRCandthrombinconcentrationintherangeof10pM–100nM,andlimitof
detection (LOD)was1.5pM.ThefabricatedAuNPs/Ab–HRP-amplified aptamerimmunosensingSPCE
strips werefurtherusedtodetectthrombininhumanserumwithalinearrangeof100pM–100nM.This
study providedthepromisingSPCEstripswithhighlysensitiveandrapiddetectionforthrombinbythe
electrochemical aptasensorcombinedwithAuNPsconjugatesforamplifyingthedetectionsignal

ผลลัพธ์ (ไทย) 1:

Thrombin playstheroleincardiovasculardiseasesandregulatesmanyprocessesininflammation และสามารถ beafeatureofmanypathologicalconditions, includingthethromboembolicdisease, cancerandneurodegenerativediseases Anultrasensitiveandamplified electrochemicalsandwichassayusingจอ printedcarbonelectrode (SPCE) stripsforthrombindetectionwasestablishedinthisstudy การการนำไฟฟ้า andsensingperformanceofthecarbonelectrodeswereenhancedbyusinggoldเก็บกัก (AuNPs) Theaptameraddressedonthestripswasusedasaprimaryprobetocapturethrombin inthedetectedsamples Anamplifier wasinventedforrecognizingthrombincapturedontheSPCE, whichisthemultiplemoleculesofanti-thrombinantibody(Ab)andhorseradishperoxidase(HRP)AuNPs(AuNPs/Ab–HRP) แก้ไขร่วมกัน HydrogenperoxidewasusedasthesubstrateforHRPandthentheการตอบสนองปัจจุบัน couldbedetected (RC) ขยาย TheoptimizationoftheseAuNPsconjugates aptamerimmunosensing SPCEstripswasconductedforthrombindetection.Thedetectionsensitivityshowedaเชิงเส้น relationbetweenRCandthrombinconcentrationintherangeof10pM – 100nM, andlimitofการตรวจจับ (LOD)was1.5pM.ThefabricatedAuNPs/Ab–HRP-amplified aptamerimmunosensingSPCEแผ่น werefurtherusedtodetectthrombininhumanserumwithalinearrangeof100pM–100nM.ThisprovidedthepromisingSPCEstripswithhighlysensitiveandrapiddetectionforthrombinbythe ศึกษาไฟฟ้า aptasensorcombinedwithAuNPsconjugatesforamplifyingthedetectionsignal

ผลลัพธ์ (ไทย) 2:

playstheroleincardiovasculardiseasesandregulatesmanyprocessesininflammation thrombin และ
สามารถ andsensingperformanceofthecarbonelectrodeswereenhancedbyusinggold อนุภาคนาโน (AuNPs) .Theaptameraddressedonthestripswasusedasaprimaryprobetocapture thrombin inthedetectedsamples.Anamplifier wasinventedforrecognizingthrombincapturedonthe SPCE, whichisthemultiplemoleculesofanti-thrombinantibody (AB) andhorseradishperoxidase (HRP) AuNPs ร่วมแก้ไข (AuNPs / Ab-HRP) HydrogenperoxidewasusedasthesubstrateforHRPandthenthe ตอบสนองปัจจุบัน aptamer (RC) couldbedetected.TheoptimizationoftheseAuNPsconjugates แอมพลิฟายimmunosensing SPCEstripswasconductedforthrombindetection.Thedetectionsensitivityshoweda เชิงเส้น relationbetweenRCandthrombinconcentrationintherangeof10pM-100nm, andlimitof การตรวจสอบ (LOD) was1.5pM.ThefabricatedAuNPs / Ab-HRP-ขยาย aptamerimmunosensingSPCE แถบ werefurtherusedtodetectthrombininhumanserumwithalinearrangeof100pM-100nM.This ศึกษา providedthepromisingSPCEstripswithhighlysensitiveandrapiddetectionforthrombinbythe ไฟฟ้า aptasensorcombinedwithAuNPsconjugatesforamplifyingthedetectionsignal