The interpretation
of forensic DNA mixtures, while routine,
is often complex, requiring careful analysis and alternative
statistical methods (13). This limitation
was only partially diminished by the introduction
of more advanced techniques into genetic typing
such as PCR-based STR-analysis (1) and single locus
probes (14). In spite of these improvements,conventional microdissection of hematoxylin and
eosin-stained sections – usually performed by a
needle or a blade – was not always successful in
diminishing the extent of maternal tissue contamination.
Consequently, co-amplification of fetal alleles
would often fail due to preferential amplification
of the abundant maternal DNA (8).