that excise the p25 CP expression cassette, or
with DraI, that cut once the T-DNA near the RB. After
electrophoresis in 1% agarose gels, the DNA was
blotted to nylon membranes and probed with a digoxigenin
(Roche)-labeled fragment of the coding region
of the p25 CP gene, prepared by PCR following the
manufacturer’s protocol