Abstract: In order to reduce our dependence on nonrenewable plastics and solve the problem of nonbiodegradable plastic waste, there has been much attention paid to the development of biodegradableplastics from natural resources. Starch-based materials have been widely studied and developed forcommercial production, primarily from corn and tapioca. However, the use of these starches couldgenerate food security problems. Therefore, the use of alternative starch sources, such as agriculturalwaste, would be of great interest. In this work, we investigated the properties of films prepared frompineapple stem starch, which has a high amylose content. Pineapple stem starch (PSS) films andglycerol-plasticized PSS films were prepared and characterized using X-ray diffraction and watercontact angle measurements. All films exhibited some degree of crystallinity, making them waterresistant. The effect of glycerol content on mechanical properties and gas (oxygen, carbon dioxideand water vapor) transmission rates was also studied. The tensile modulus and tensile strength of thefilms decreased with increasing glycerol content, while gas transmission rates increased. Preliminarystudies showed that coatings made from PSS films could slow down the ripening process of bananasand extend their shelf life.