To evaluate the effect of PGE on spore germination and germ
tube elongation, 12.5 ml of a conidia suspension (106 conidia/ml)
were transferred to Eppendorf tubes and mixed with an equal
volume of potato dextrose broth (PDB, Sigma–Aldrich) at strength
of 24 g/land 25 ml of 2X PGE solutions. In control samples
pomegranate extract was replaced by citric acid at 1%
final
concentration.