H2S donors cause stomatal opening in Capsicum anuum. The leaves of
analyzed from Capsicum anuum plants which were between 6 and 7 weeks old.
Stomatal bioassays were performed as described previously by Desikan et al.9
Epidermal peels were incubated in MES-KCl buffer [10 mM 2-morpholino ethane
sulfonic acid (MES), 5 mM KCl, 50 μM CaCl2, pH 6.15] for 2.5 h exposed to the
direct lightning (in 60–100 lE m-2 s-1) before the addition of various compounds.
(A) Samples were sheltered from direct lighting and treated with ABA or NaHS
for 2.5 h and left under the day light conditions before stomata apertures were
analyzed. (B) Samples were sheltered from direct lighting and treated with ABA
or GYY 4137 for next 2 h and left under the day light conditions before stomata
apertures were analyzed. Apertures were measured using a light microscope and
imaging camera with LEICA QWIN image processing and analysis software (Leica
Microsystems and Imaging Solutions, Cambridge, UK). n = 40 stomatal apertures,
±SE. GYY4137 was synthesis as previously described in reference 7.