Another probe for in situ hybridization gives good
results with both GAVand with virulent and non virulent
forms of YHVin Thailand (Fig. 14) (Soowannayan et al.,
2003). It comprised a 794 bp RT-PCR labeled fragment
prepared using virulent YHV from Thailand as the
template and targeting the ORF 1b region of the viral
genome. The primers used were:
Sense: 5′-GAC ATC ACT CCA GA-3′
Antisense: 5′-GTG AAG TCC ATG TGT GTG