Recent work has shown how important the role that the
so-called accessory enzymes can play in the effective enzymatic
hydrolysis [5–8]. These accessory enzyme activities can
improve the performance of enzyme preparations even when
not directly involved in the hydrolysis of cellulose. For example,
-glucosidase activity is of uttermost importance to avoid accumulation
of cellobiose and thus severe inhibition of the cellulases
[9–11]. Hemicellulases and pectinases can hydrolyze their respective
non-cellulosic polysaccharide substrates that coat cellulose
fibers, and thus assist in the hydrolysis of cellulose to boost a
release of fermentable sugars from lignocellulosic biomass [4,12].
A xylanase-boosting effect has been observed on a range of pretreated
lignocellulosic materials, regardless of their xylan content
[7]. Another newly found variety, lytic polysaccharide monooxygenases
(LPMOs) Auxiliary Activities Family 9 (AA9, formerly known