Weigh accurately 200 mg of the sample into a 25mL volumetric flask. Dissolve it in a smallvolume of 1-butanol and make up to volume with same solvent.2. Transfer, using a pipette 5.0 ml of sample solution to a dry test tube; add by pipette 5.0 ml ofthe TBA reagent solution. Close the test tube and mix thoroughly.3. Place the prepared test tube into a water bath at 95°C for 30 min (120 min). Remove the testtube and cool it under running tap water until it reaches room temperature.4. Measure the absorbance of the reaction solution at 530 nm using distilled water as a blank toset zero. Run a reagent blank and sample