The moderately halophilic, cultivable fraction of prokaryotes thriving in hypersaline sediments of a solar
saltern in Mallorca, Spain, has been studied by means of different cultivation media. A set of 374 isolates
retrieved with six different culture conditions was screened, using whole-cell MALDI-TOF MS analysis
to classify them into 25 phenotypic clusters at 52% similarity. The phylogenetic inference, made from
comparative sequence analyses of the 16S rRNA genes of selected strains, indicated that each phenotypic
cluster was comprised of a genealogically homogeneous set of strains. DNA–DNA hybridization (DDH)
results among selected strains confirmed that each MALDI-TOF cluster encompassed members of the
same species. On the other hand, the intra-cluster diversity, measured by several RAPD (Random Amplified Polymorphic DNA) amplifications, indicated that the clusters corresponded to several populations
of the same phylogenetic unit coexisting in the same environment. The results encourage the use of
MALDI-TOF MS for further exhaustive studies of the cultivable diversity of hypersaline environments.
The moderately halophilic, cultivable fraction of prokaryotes thriving in hypersaline sediments of a solarsaltern in Mallorca, Spain, has been studied by means of different cultivation media. A set of 374 isolatesretrieved with six different culture conditions was screened, using whole-cell MALDI-TOF MS analysisto classify them into 25 phenotypic clusters at 52% similarity. The phylogenetic inference, made fromcomparative sequence analyses of the 16S rRNA genes of selected strains, indicated that each phenotypiccluster was comprised of a genealogically homogeneous set of strains. DNA–DNA hybridization (DDH)results among selected strains confirmed that each MALDI-TOF cluster encompassed members of thesame species. On the other hand, the intra-cluster diversity, measured by several RAPD (Random Amplified Polymorphic DNA) amplifications, indicated that the clusters corresponded to several populationsof the same phylogenetic unit coexisting in the same environment. The results encourage the use ofMALDI-TOF MS for further exhaustive studies of the cultivable diversity of hypersaline environments.
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ชอบเกลือปานกลางส่วนของการเพาะปลูก The moderately halophilic, cultivable fraction of prokaryotes thriving in hypersaline sediments of a solar
saltern ชุด saltern in Mallorca, Spain, has been studied by means of different cultivation media. A set of 374 isolates
ไอโซเลทที่ดึงหกเงื่อนไขวัฒนธรรมที่แตกต่างได้รับการคัดเลือกโดยใช้ทั้งการวิเคราะห์เซลล์MALDI retrieved with six different culture conditions was screened, using whole-cell MALDI-TOF MS analysis
ที่จะแยกพวกเขาออกเป็น to classify them into 25 phenotypic clusters at 52% similarity. The phylogenetic inference, made from
comparative sequence analyses of the 16S rRNA genes of selected strains, indicated that each phenotypic
cluster was comprised of a genealogically homogeneous set of strains. DNA–DNA hybridization (DDH)
results among selected strains confirmed that each MALDI-TOF cluster encompassed members of the
same species. On the other hand, the intra-cluster diversity, measured by several RAPD (Random Amplified Polymorphic DNA) amplifications, indicated that the clusters corresponded to several populations
of the same phylogenetic unit coexisting in the same environment. The results encourage the use of
MALDI-TOF MS for further exhaustive studies of the cultivable diversity of hypersaline environments.
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