Chestnut media and chestnut extract were even prepared using
flours obtained from milling of chestnuts produced in Emilia
Romagna region (North Italy) and Calabria region (South Italy). In
detail, chestnut flour from Northern Italy (protein: 7.05%; lipid:
6.94%; carbohydrate: 60%) was purchased from “Molino Spadoni
S.p.A.” (Ravenna), whereas flour from Southern Italy (protein: 6.1%;
lipid: 3.7%; carbohydrate: 76.2) was purchased from ‘‘Antichi Sapori”
(Catanzaro). Tests to assess microbial viability during transit in
simulated gastric juice were performed as previously described by
using strain LbGG.