The DPPH
solution (1 mL) was added to 1 mL of centrifuged supernatant with 3
mL of ethanol. The mixture was shaken vigorously and allowed to
stand at room temperature in the dark for 10 min. The decrease in
absorbance was measured at 517 nm using a Shimadzu UV-2401 PC
spectrophotometer. Ethanol was used as blank. All determinations were
performed in triplicate. The results of the assay were expressed relative
to micromolar Trolox per 100 g of dry weight