Shake¯askfermentation wasconductedin triplicate
with mixedsugars (80 g/l®nal,40g/leachxyloseand
glucose). Erlenmeyer¯asks(125ml)containing50mlof
the fermentation mediumwereinoculated with5mlof
various cell suspensions. Inocula wereprepared by
washingcellsfromthesurfacesof2-day-oldYEPXagar
plate cultures. Cell inocula were adjusted to 2.5 g/l.
Fermentation¯askswereshakenat100rpmat25±27°C.
Fermentationwasmonitoredfor3±5daysbyremoving
1.3 mlsamplesfor sugarandethanolanalyses