The loss of isoflavones with the soaking water as
estimated by the differences in the amount detected in
raw soybeans and soaked soybeans was 4%. The total isoflavone content of the slurry (550 mg) was lower
than that of the raw soaked soybeans (690 mg) (Table
2) and a change in distribution of isoflavone components
was observed (Table 3). Grinding of soaked-soybean
in boiling water seems to result in the major loss
of isoflavones during processing.