Previous reports have demonstrated that the lipid production by oleaginous microorganisms is heavily influenced by the carbon-to-nitrogen (C/N) molar ratio As shown in Figure 3A, when the (NH4)2SO4 was increased from 0.1 to 10 g/l corresponding to a decrease of the C/N ratio from 200 to 2, the optical density increased from 2.1 to approximately 4.0. After 36 hrs of cultivation, higher amounts of nitrogen led to an increase in the biomass, with the highest cell dry weight of 0.89 g/l obtained when cells were cultivated in synthetic wastewater containing an initial (NH4)2SO4 concentration of 10 g/l (Figure 3A).