In each SFE step, the extract was collected in acetone, and
the preparative procedures of the sample solution for UV and
HPLC analysis were same as those for the organic solvent
extraction. The extraction efficiency of SFE was compared
with that of conventional solvent extraction using organic
solvent such as acetone.
Analysis of astaxanthin was carried out by high performance
liquid chromatography (HPLC) (Samsung, Korea)
with an SLC-200 UV/VIS detector. Sample solution was
injected through a 20l loop and separated on the Phenomenex
Luna-Silca column (3 m silca, 4.6mm×150mm)
at ambient temperature. Isocratic elution was performed with