With consideration of sustainable development, this study explored the fermentation strategy of
cost-effective production of biodegradable polymer- polyhydroxyalanoates (PHAs) for feasibility of ecofriendly
materials recycling during wastewater treatment. As prior studies showed that Aeromonas
hydrophila NIU01 was a promising PHA-producing bacterium, this follow-up study tended to seek for
optimal nutrient-supplementation strategy to stimulate maximal PHA accumulation of A. hydrophila
NIU01 for cellular production. As maximal PHA production took place at growth-limiting conditions,
two-stage fermentation was much more appropriate for practical applications compared to batch mode
of operation. Moreover, this optimal two-stage operation strategy maximized cellular PHA production
under nitrogen-limiting conditions at C/N molar ratio of 60/1. For materials recycling, this operation
strategy could be applicable to simultaneous PHB production and wastewater decolorization using A.
hydrophila.