isopropanol and ammonium sulfate precipitation, hydrophobic interaction chromatography (HIC) and size exclusion adapted from Diogo et al.(2000). Briefly, cells were suspended in 110 mL of 50 mM glucose,10 mM EDTA, and 25 mM Tris pH 8.0. Lysis was performed byadding 110 mL of a 200 mM NaOH, 1% (w/v) sodium dodecyl sul-fate (SDS) solution on ice and stopped by the addition of 110 mL of apre-