2.2.3. Needle-type enzyme sensor
A tube-shaped immobilized enzyme membrane was inserted
into the tip of the needle-type hollow container (Fig. 1C). The
container was soaked in 0.1MPBS (pH 7.0) so that the immobilized
enzyme membrane would swell and adhere to the needletype
hollow container. The container was also lightly tapped
to eliminate air bubbles. Next, the optic fiber probe including
a ruthenium complex (FOXY-AL300: Ocean Optics, FL,
USA) was inserted inside the immobilized enzyme membrane
(Fig. 1D) to set up the needle-type glucose sensor.