3.4. Viscosity of modified XGs
The viscosities of tamarind seed XG following depolymerization with endo-(1→4)-β-d-glucanase are shown in Fig. 3. When the molecular weight of tamarind seed XG was reduced to that of unmodified apple pomace XG, the viscosity was reduced from approximately 85 to 7 mPa·s at 1% (w/v); unmodified apple pomace XG had a viscosity of 8 mPa·s when tested at the same concentration. Similarly, when the molecular weight of tamarind seed XG was reduced to that of unmodified Nicotiana XG, the viscosity was reduced from approximately 85 to 3 mPa·s at 1% (w/v), compared with 2 mPa·s for unmodified Nicotiana XG tested at the same concentration.Apple pomace XG at 1% (w/v) gave solutions which displayed near-Newtonian behaviour and had a viscosity of approximately 8 mPa·s (Fig. 2B). Following enzymic removal of terminal Fucp residues (from 7 to 1 mol%), the viscosity at 1% (w/v) was approximately 4 mPa·s, similar to apple pomace XG at 0.5% (w/v). Following enzymic removal of terminal Galp residues (from 6 to 1 mol%) the solution became turbid. MALLS gave large molecular weights (>1000 kDa) for defucosylated, degalactosylated apple pomace XG suspension, indicative of significant aggregation in this state. The viscosity of this defucosylated, degalactosylated apple pomace XG suspension (1% w/v) was approximately 5 mPa·s.
Native Nicotiana XG at 1% (w/v) gave clear solutions, which displayed near-Newtonian behaviour and had a viscosity of approximately 2 mPa·s ( Fig. 2C). Following deacetylation, the Nicotiana XG solution also became turbid and the viscosity (1.5 mPa·s at 1% w/v) was essentially unaltered. Similar to apple pomace XG following removal of Fucp and the Galp residues, MALLS gave large molecular weights of the order of 1000 kDa for deacetylated Nicotiana XG suspended in water, indicative of significant aggregation.
3.4. Viscosity of modified XGs
The viscosities of tamarind seed XG following depolymerization with endo-(1→4)-β-d-glucanase are shown in Fig. 3. When the molecular weight of tamarind seed XG was reduced to that of unmodified apple pomace XG, the viscosity was reduced from approximately 85 to 7 mPa·s at 1% (w/v); unmodified apple pomace XG had a viscosity of 8 mPa·s when tested at the same concentration. Similarly, when the molecular weight of tamarind seed XG was reduced to that of unmodified Nicotiana XG, the viscosity was reduced from approximately 85 to 3 mPa·s at 1% (w/v), compared with 2 mPa·s for unmodified Nicotiana XG tested at the same concentration.Apple pomace XG at 1% (w/v) gave solutions which displayed near-Newtonian behaviour and had a viscosity of approximately 8 mPa·s (Fig. 2B). Following enzymic removal of terminal Fucp residues (from 7 to 1 mol%), the viscosity at 1% (w/v) was approximately 4 mPa·s, similar to apple pomace XG at 0.5% (w/v). Following enzymic removal of terminal Galp residues (from 6 to 1 mol%) the solution became turbid. MALLS gave large molecular weights (>1000 kDa) for defucosylated, degalactosylated apple pomace XG suspension, indicative of significant aggregation in this state. The viscosity of this defucosylated, degalactosylated apple pomace XG suspension (1% w/v) was approximately 5 mPa·s.
Native Nicotiana XG at 1% (w/v) gave clear solutions, which displayed near-Newtonian behaviour and had a viscosity of approximately 2 mPa·s ( Fig. 2C). Following deacetylation, the Nicotiana XG solution also became turbid and the viscosity (1.5 mPa·s at 1% w/v) was essentially unaltered. Similar to apple pomace XG following removal of Fucp and the Galp residues, MALLS gave large molecular weights of the order of 1000 kDa for deacetylated Nicotiana XG suspended in water, indicative of significant aggregation.
การแปล กรุณารอสักครู่..
