The seedlings were subjected to 150 mM sodium chloride
(NaCl) treatment by supplying salt to the nutrient
solution after 21 days. Next day, it was replaced with a
new half-strength Hoagland’s nutrient solution that contained
250 mM NaCl. Subsequently, cv. R. and cv. W.
were investigated for their responses to NaCl stress.
Compared with the controls, analyses were done at 3, 6, 12,
24, 48 h after treatment. A part of mixed roots (5-cm
section from root tip) were treated and used for MDA
determination and the others were frozen in liquid nitrogen
and kept at -80C for further antioxidant enzymes
analysis.