For the determination of ability to chelate copper in the enzyme of
isolated compounds, the mixture consisting of 1800 ml of 0.1 M
phosphate buffer (pH 6.8), 1000 ml of water, 100 ml of 0.05 mM
sample solution and 100 ml of the aqueous solution of the
mushroom tyrosinase (138 units) was incubated at 25°C for 30 min,
and then the spectra were recorded.