The whole liver of each rat were excised after euthanasia, rinsed with 0.9% NaCl-injectable solution, blotted dry with tissue paper, and weighed.
Two pieces of liver tissues (0.5×0.5×0.5 cm for each) were collected from both left half and right half livers from each rat.
Liver tissues were fixed in 10% neutral phosphate-buffered formalin, embedded in paraffin, sectioned at 4-μm/slide, and then processed for routine histological examination with hematoxylin-eosin staining.
The whole liver of each rat were excised after euthanasia, rinsed with 0.9% NaCl-injectable solution, blotted dry with tissue paper, and weighed. Two pieces of liver tissues (0.5×0.5×0.5 cm for each) were collected from both left half and right half livers from each rat. Liver tissues were fixed in 10% neutral phosphate-buffered formalin, embedded in paraffin, sectioned at 4-μm/slide, and then processed for routine histological examination with hematoxylin-eosin staining.
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