This experiment was conducted to evaluate the effects of different levels of Portulaca oleracea (PO), medicinalplant, on performance, egg traits, blood biochemical and immunity parameters of laying hens. Onehundred eighty Hi-Line (W-36 strain) mature laying hens 65 up to 76 weeks of age were allotted in a completelyrandomized design consisting of 5 treatments and 3 replicates (12 birds per replicate). Treatmentgroups consisted of a control group (1) with no PO supplementation and experimental groups 2, 3, 4 and 5which contained 0.5%, 1.0%, 1.5% and 2.0% of PO powder, respectively. Using different levels of PO significantlyaffected the performance, egg traits, blood biochemical and immunity parameters of laying hens(P<0.05). The highest values for egg weight, egg production percentage, egg mass, feed intake, feed conversionratio, egg yolk color index, albumin percentage and blood high density lipoprotein (HDL) wereobtained by feeding 2.0% of PO. The highest level of blood hemoglobin was observed by feeding 1.0% ofPO. The overall results showed that feeding Portulaca oleracea powder up to 2.0% in the diet has a positiveeffect on mature laying hens performance and egg traits.
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