Chlorella species were grow in flask containing enrichment culture medium for 7 days and the biomass separated was inoculated in 1 liter and 5 liter capacity rectangular open bioreactor and incubated at ambient temperature in chamber with light sources . Developed biomass was then cultivated in larger volumes in photobioreactor as well as open ponding system at thermocol molding factory using exhaust fume using same conditions.
Biomass was allowed to settle and the sediment collected was centrifuged and dried at 105 ๐C. Dried biomass was analysed for TOC (Dichromate reflux method) Protein (colorimetric method), Lipid contents (Soxhlet extraction) and Calorific value (using Bomb calorimeter) as given in Standard Methods for Water and Waste Water Analysis 1989 [15].
Blending with coal was carried out to prepare mixture with 25 and 50% coal and calorific values were determined. Sample processing for determination of calorific value of Chlorella sp. Was done by drying them at 80 ๐C for 24 hours and recording their dry weight. The dried samples were then ground into a fine power using a mortar and pastel, and placed in desiccators. The calorific content of dried sample was measured using a Phillipson Microbomb Calorimeter. Small of dried sample (15-30 mg) were placed in the bomb chamber, pressurised to 425 psi with pure oxygen, combusted and amount of heat generated recorded, The Calorimeter was calibrated against benzoic acid standards [16]. Dried biomass of chlorella was subjected to hydrothermal liquefaction tonrecover oil and the same was anailsed for oil contents along with GCMS and FTIR analysis to determine the chemical and physical properties of oil.