This is due to our observation that tagged-Htt when over expressed in human embryonic stemcells (hESCs) fails to
localize to mitochondria, thus suggesting a non-autonomous effect (data notshown). Regardless of the mechanism how ever, this aspect of Htt function on mitochondria membrane potential was unexpected and previously unnoticed. The construction of syngeneic htt/, and gain-ofpoly-Qmutants, currently in progress in
hESCs, and their comparison with wild-type cells, will allow
performing the same analysis directly in humans
This is due to our observation that tagged-Htt when over expressed in human embryonic stemcells (hESCs) fails tolocalize to mitochondria, thus suggesting a non-autonomous effect (data notshown). Regardless of the mechanism how ever, this aspect of Htt function on mitochondria membrane potential was unexpected and previously unnoticed. The construction of syngeneic htt/, and gain-ofpoly-Qmutants, currently in progress inhESCs, and their comparison with wild-type cells, will allowperforming the same analysis directly in humans
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