A novel and rapid green non dispersive ionic liquid based microextraction method has been developed to quantify the trace levels of Cu2+ in ASE of different SLT product. A long narrow glass column was used to provide more contact area between aqueous and extractive medium, which considerably improve phase transfer ratio of analyte from aqueous to extractive medium, to obtained high enrichment factors. The high tolerance of coexisting ions and excellent analytical performance shows that it is a good alternative for the determination of trace Cu2+ level in complex matrixes. The content of Cu2+ extracted by artificial saliva is immediately available, absorbed and affecting the lining of mouth. The artificial saliva extracted Cu2+ corresponds to 30–50%, of total content of Cu2+ in different types of SLT products (gutkha, mainpuri and dry snuff). The Cu2+ levels in SLT and its contribution to the daily intake was established, the consumption of 10 g of different types of SLT products provides 0.4–0.9% of the PMTDI for Cu2+ in adults. We consider that consumption of studied SLT products, especially those consuming more than 10 g per day, are at risk of developing ill health.