Meristem culture has become a powerful and successful tool for virus elimination from infected plants
and has been successfully applied in potato. A total of 70 potato (Solanum tuberosum L.) tubers for each of
three cultivars that grown in Jordan were obtained from the Ministry of Agriculture and subjected to
enzyme-linked immunosorbent assay (ELISA) test for detection of virus infection. The percentage of infection
with Potato virus Y (PVY) was 21.4%, 15.7 %, 12.8% for Spunta, Alaska and Safrane cultivars, respectively.
Meristem culture was applied on infected potato tubers for all the three cultivars. Results of shoots and roots
development indicated that medium supplemented with 0.5 mg/l of Indole butyric acid (IBA) was the best for
shoots length with mean (7.71 cm), roots length with mean (9.41 cm), number of shoots with mean (2.60) and
number of leaves with mean (15.40). ELISA results for in vitro produced plantlets showed that all tested
plantlets were PVY free. Reverse transcription polymerase chain reaction (RT-PCR) was successfully amplified
the coat protein gene of PVY virus in the infected samples and confirm the ELISA result. The Acclimatization
of the plantlets that generated from in vitro shoots and roots multiplication stage revealed 90% of successful
with Spunta cultivar and 80% with both Alaska and Safrane cultivars.