We report the use of chemical derivatization with MALDI-MS/MS analysis for de novo sequence
analysis. Using three frequently used homology-based search algorithms, we were able to identify
more than 40 proteins from banana, a nonmodel plant with unsequenced genome. Furthermore, this
approach allowed the identification of different isoforms. We also observed that the identification score
obtained varied according to the position of the peptide sequences in the query using the MS-Blast
algorithm.