Since this is the first of this kind of study with cooking oil media, no publications could be located in the existing literature and hence this was taken as a pilot study. A total of 200 patients satisfying the criteria for recruitment were randomly allocated to two groups. Block randomization was done with 5 blocks each having 40 cases for allocation. This was done just to avoid the non-availability of required sample size and to keep randomization live with the available number of patients. However, 200 patients were available at the end of study.